Table 1.
Cross-reactivity of nanobody based PolyHRP ELISA for human sEH with sEH of other species and other human epoxide hydrolases.
| Epoxide hydrolases (puritya) | Cross-reactivity (%) | Epoxide hydrolases (purity) | Cross-reactivity (%) |
|---|---|---|---|
| Human sEH (>95%) | 100 | Dog sEH hepatic S9 (~20%) | <0.1 |
| Mouse sEH (>95%) | 1.0 | Liver cytosol from sEH KO mice | <0.1 |
| Rat sEH (>95%) | 2.4 | Human mEH (~80%) | <0.1 |
| Denatured human sEH (>95%) | 0.8 | Human EH3 (~4%) | <0.1 |
| Horse sEH hepatic S9 (~20%) | <0.1 | -- | -- |
a
purity was determined by densitometry on SDS-PAGE using recombinant affinity purified enzyme except for equine and canine enzyme partially purified by ion exchange chromatography from liver homogenates.