Table 2.
Recovery of pure human sEH spiked into the hepatic cytosol samples from sEH knockout mice (n = 3)a.
| spiked (ng/mL) | 1:100 dilution | 1:500 dilution | 1:1000 dilution | |||
|---|---|---|---|---|---|---|
| detected (ng/mL) | recovery | detected (ng/mL) | recovery | detected (ng/mL) | recovery | |
| 0.00 | 0.78 ± 0.09 | -- | 0.16 ± 0.00 | -- | 0.06 ± 0.00 | -- |
| 0.25 | 0.96 ± 0.08 | 74% | 0.36 ± 0.01 | 83% | 0.41 ± 0.06 | 142% |
| 0.50 | 1.19 ± 0.02 | 84% | 0.64 ± 0.11 | 96% | 0.61 ± 0.06 | 111% |
| 1.00 | 1.59 ± 0.06 | 81% | 0.96 ± 0.01 | 81% | 1.26 ± 0.03 | 120% |
| 2.00 | 2.46 ± 0.16 | 84% | 2.03 ± 0.07 | 94% | 1.99 ± 0.16 | 97% |
| 4.00 | 4.43 ± 0.36 | 91% | 3.72 ± 0.11 | 89% | 4.08 ± 0.17 | 101% |
| 6.00 | 5.82 ± 0.41 | 84% | 5.30 ± 0.31 | 86% | 5.50 ± 0.26 | 91% |
| 8.00 | 7.33 ± 0.82 | 82% | 6.96 ± 0.56 | 85% | 7.48 ± 0.45 | 93% |
a
sEH activity was not detected in unspiked liver cytosol samples from sEH KO mice as described in ref 39.