. Author manuscript; available in PMC: 2018 Jun 6.

Published in final edited form as: Anal Chem. 2017 May 10;89(11):6248–6256. doi: 10.1021/acs.analchem.7b01247

Table 3.

Comparison of PolyHRP ELISA and four other methods for the analysis of human sEH in the S9 fraction of pooled (4–50 persons) human tissues samples.

Human tissues	sEH concentration (nM) estimated^a
Human tissues	Enzyme activity^b	Western blot^b	Conventional ELISA^c	ELISA NbS7 /NbS43^d	PolyHRP ELISA
Liver	420 ± 89 (21%)	500 ± 150 (30%)	386 ± 48 (12%)	468 ± 22 (5%)	691 ± 18 (3%)
Kidney	44 ± 3 (7%)	80 ± 15 (19%)	44.4 ± 1.6 (4%)	37 ± 3 (8%)	76.3 ± 0.7 (1%)
Lung (nonsmoker)	3.0 ± 0.3 (10%)	22 ± 6 (27%)	6.1 ± 0.3 (5%)	2.9 ± 0.1 (3%)	3.60 ± 0.12 (3%)
Lung (smoker)	2.8 ± 0.3 (11%)	21 ± 4 (19%)	5.9 ± 0.1 (2%)	2.1 ± 0.2 (10%)	4.07 ± 0.03 (1%)
Intestine	80 ± 9 (11%)	110 ± 10 (9%)	--	121 ± 6 (5%)	131 ± 2.5 (2%)
Renal	--	--	--	--	44.2 ± 0.4 (1%)

Results are average ± SD (CV, n=3); 1 nM sEH corresponds to 62.5 ng/mL sEH.

^b,c,d

Data from ref 40, 29, 30, respectively.