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. 2017 Sep 20;8:1151. doi: 10.3389/fimmu.2017.01151

Figure 5.

Figure 5

Toll pathway regulates the expression of AMPs via the transcription factor Dorsal. (A,B) AMP expression was detected in hemocytes of shrimp after challenge with Staphylococcus aureus (A) or Vibrio anguillarum at 6 h (B). (C) The RNAi efficiency of Dorsal, Toll1, Toll2, and Toll3 was analyzed by qPCR. (D,E) AMP expression was detected in Dorsal-knockdown shrimp challenged with S. aureus (D) or V. anguillarum (E). (F,G) AMP expression was detected in Toll1-knockdown shrimp challenged with S. aureus (F) or V. anguillarum (G). (H,I) AMP expression was detected in Toll2-knockdown shrimp after challenge with S. aureus (H) or V. anguillarum (I). (J,K) AMP expression was detected in Toll3-knockdown shrimp challenged with S. aureus (J) or V. anguillarum (K). β-Actin was used as internal control. Significant differences are indicated with asterisks (*p < 0.05, **p < 0.01).