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. 2017 Sep 20;8:1604. doi: 10.3389/fpls.2017.01604

FIGURE 2.

FIGURE 2

TAG content in GmLEC2a over-expression Arabidopsis seeds. Ectopic expression of GmLEC2a in Arabidopsis alters TAG composition and enhance TAG content in mature seeds. (A) Expression of GmLEC2a in atlec2 mutant seeds by semi-qRT-PCR. (B) Composition of fatty acids in seeds of Arabidopsis atlec2, wild-type WS-2, and GmLEC2/atlect2 complementation plants. (C) Composition of fatty acids in seeds of Col-0 and GmLEC2aOE. (D) Over-expression of GmLEC2a in Col-0 (GmLEC2aOE) detected by using semi-qRT-PCR. (E) TAG contents in seeds of Arabidopsis atlec2, wild-type WS-2, and GmLEC2/atlect2 complementation plants. (F) TAG content in seeds of wild-type Col-0 and GmLEC2a overexpression (GmLEC2aOE) plants. Transcript levels are expressed relatively to that of AtACTIN. All data are three biological replicates and are expressed as means ± SD. ∗∗P < 0.01 and ∗P < 0.05 by Student’s t-test (n = 3). Asterisks indicate the significant difference relative to the atlec2 mutant.