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. 2017 Sep 20;8:1604. doi: 10.3389/fpls.2017.01604

FIGURE 4.

FIGURE 4

Ectopic expression of GmLEC2a in soybean hairy roots. (A,B) Hairy roots over-expressing GmLEC2a and GUS. The cotyledons of germinating soybean seeds were used for infection with Agrobacteria K599 harboring pB2GW7-GmLEC2a or GUS gene (control). The generated hairy roots over-expressing GUS (A) and GmLEC2a (B) were selected on MS medium containing ppt. (C,D) TLC analysis of neutral lipids extracted from hairy root over-expressing GmLEC2a (D) and GUS control (C). (E) qRT-PCR conformation of GmLEC2a or GUS gene expression. Transcript levels are expressed relatively to that of GmACTIN. (F,G) Comparison of TAG composition (G) and content (F) in hairy roots over-expressing GmLEC2a and GUS control grown and extracted under the identical conditions. More than 5 independent transgenic hairy root lines were analyzed. Data are from three biological replicates. All data are three biological replicates and are expressed as means ± SD. ∗∗P < 0.01 and ∗P < 0.05 by Student’s t-test (n = 3).