Figure 1.

N-Methyl-D-Aspartate (NMDA)-injected animals show reduction in number of retinal ganglion cells (RGCs) and consequent visual function deficits. (A–F) Brn3a whole mount retina immunostaining showing reduced number of Brn3a positive cells in NMDA-injected animals compared to control animals. (G) Graph represents quantitative analysis showing the percentage of Brn3a positive cells. (H–I) Schematic of Optomotry and light avoidance test set up. (J) Visual acuity of NMDA-injected animals is significantly low compared to that of control animals. (K) NMDA-injected animals also showed less light avoidance behavior compared to that of control animals. Data are expressed as Mean ± SD. Number of animals used for behavioral experiments; Control = 10 and NMDA injected = 10. Scale = 100 μm.