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Promoter transient assays in N. benthamiana. (A,B) Agrobacterium carrying promoter-reporter constructs pOsPR10a::GFP-GUS or pCHIT2::GFP-GUS were co-infiltrated into leaves along with Agrobacterium carrying an effector construct (35S::OsWRKY88). Infiltrated leaves were removed at 24 hpi and visualized by GUS staining. (C,D) Infiltrated leaves used to measure GUS enzyme activity.
