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. 2017 Sep 20;10:296. doi: 10.3389/fnmol.2017.00296

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Copyright © 2017 Garcia, Formoso, Aparicio, Frasch and Scorticati.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Endocytosed M6a is sorted to both late and recycling endosomes. M6aRFP/Rab7- or Rab11-coexpressing neurons and HEK293 cells were subjected to the antibody internalization assay (AIA). (A,B) Representative confocal images from M6aRFP/Rab7GFP-overexpressing cells at T0 (steady state) and T1 (1 h after immunouptake). (C,D) Representative confocal images from M6aRFP/Rab11GFP-overexpressing cells at T0 and T1. M6aRFP is shown in green and RabGTPases are shown in magenta. Conditions in which dots exhibited colocalization between endocytosed M6a and the corresponding endocytic compartments are shown in white in the inset of merge images and are plotted for HEK293 cells (for statistical data see Supplementary Table S1). Scale bar: 15 μm. Inset for neurons: 5 × 5 μm; inset for HEK293: 4 × 4 μm.