Figure 2. Workflow for an integrated analysis of chemical genomic and proteomic data obtained from a single biological sample of CSCs. The first step is to efficiently isolate and enrich tumor-initiating cells from diverse tumor types or established cancer cell lines. These biological samples are then lysed. DNA, RNA, and proteins are then extracted from a single sample in different phases. This workflow provides all-in-one analysis of genome, transcriptome, and proteome from a single biological sample.