Figure 2. NK cell-dependent effects of TSA in a HCC xenograft mouse model. (a) Schematic plot of the study design and route of injection for therapeutic efficacy. (b) Representative dot plot images showing abrogation of LY6G+ cells and (c) MHC-II+ F4/80+ macrophages in Balb/c nude mice following anti-asialo-GM1 antibody injection. (d) Representative dot plot images showing abrogation of CD49b+ NK1.1+ NK cells in Balb/c nude mice following anti-asialo-GM1 antibody injection. (e) The mice were depleted of NK cells, using the anti-asialo-GM1-antibody, or were injected with IgG isotype antibody prior to, and after, tumor cell implantation, as described in Materials and Methods (n=6 mice). Tumor size was measured at the indicated days. (f) Tumor weight of each group of mice (n=6) at day 11 of the experiment. (g) Number of NK cells in the tumor tissues of mice (n=6).