Figure 1. Location of the pyrosequencing assay within the RASSF1A (A) and MGMT (B) genes used for analysis of CpG methylation status. Position of the analyzed CpG sites is shown with absolute genomic coordinates according to the human genome GRCh37/hg19 assembly. Positions of the pyrosequencing assays on the chromosome 3 (A) and chromosome 10 (B) are indicated by the yellow bar. Translation start is indicated with the arrow above the ATG start codon (A).
