Figure 6. miR-382 and miR-410 directly target MMP16 3’UTR, while miR-200b targets both MMP16 and TIMP2 3’UTR. A schematic representation of the predicted seed regions in the MMP16 and TIMP2 3’UTRs (A). Dual luciferase assays of Hek293T cells co-transfected with miR-377, miR-382, miR-410 and miR-200b mimics and luciferase reporters containing matching sequence of MMP16 3’UTR subcloned in sense or anti-sense orientation (B-E), and dual luciferase assay of cells co-transfected with miR-200b mimic and vectors containing matching sense or anti-sense 3’UTR of TIMP2 (F). A normalized firefly luciferase activity (firefly luciferase activity/Renilla luciferase activity) was calculated. The values were normalized to a relative luciferase activity from cells transfected with non-targeting control mimic. Error bars represent SD of three independent replicates in a representative experiment. *p<0.05, **p<0.01, ***p<0.001 by Student’s t-test.