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. 2017 Sep 20;8:1603. doi: 10.3389/fpls.2017.01603

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Copyright © 2017 Kumar, Goswami, Shamim, Mishra, Jain, Singh, Singh, Dubey, Singh, Rai, Singh, Pathak, Chinnusamy and Praveen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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2-DE protein profiling of different tissues of wheat cv. HD2967 under normal (26 ± 2°C) and terminal HS (36 ± 2°C) conditions. (a) Gel of leaves under control condition, (b) Gel of leaves under HS, (c) Gel of stem under control condition, (d) Gel of stem under HS, (e) Gel of spike under control condition, (f) Gel of stem under HS. Leaves, stem and spike were used for the 2-DE; IPG strip of broad range pH from 3.0 to 10 were used for the IEF; RuBisCO clean-up kit was used in case of leaves sample during sample preparation.