Table 1.

List of primers used for the expression analysis of genes associated with photosynthesis (source) and starch biosynthesis pathway (sink) in wheat under normal (26 ± 2°C) and terminal HS (36 ± 2°C) conditions^*.

Primers	Primer sequence (5′–3′)	Tm (°C)
qRca-F	TACGACATCTCCGATGACCA	60.0
qRca-R	CTCGTAGGAGCTCAGGATGG	59.9
qRbcS-F	GCCGATTGAGGGTATCAAGA	60.0
qRbcS-R	CGAAGCCAACCTTGCTAAAC	59.8
qAGPase-F	CGCAGAGAAACCAAAAGGAG	59.9
qAGPase-R	GAAATTGCTCACGGAGAAGC	59.9
qSSS-F	TGCAAGCTGAAGTTGGATTG	59.9
qSSS-R	CCGGTATGGCTGCAATTAGT	59.9
qSBE-F	AGGAGCAAACGGCTGAAGTA	60.0
qSBE-R	TCCTGGTTTTGGGACAACTC	59.4
β-Act-F	GCGGTCGAACAACTGGTATT	58.4
β-Act-R	GGTCCAAACGAAGGATAGCA	58.4

^*q, Quantitative; f, forward; r, reverse; Rca, RuBisCO activase; RbcS, RuBisCO (small subunit); AGPase, ADP Glucopyrophosphorylase; SSS, soluble starch synthase; SBE, starch branching enzyme; Act, actin, quantitative real-time PCR was used for the expression profiling.