FIGURE 5.

${\mathrm{NH}}_{\mathrm{4}}^{\mathrm{+}}$-dependent transcriptional changes in TaAMT1;1 genes. Seventeen-day-old seedlings grown as described in the section “Materials and Methods” were transferred to 0.5 mM (NH₄)₂SO₄ containing solution (pH 5.5). Total roots and leaves of 17-day-old plants were sampled at 0, 1, 3, and 6 h after the addition of ${\mathrm{NH}}_{\mathrm{4}}^{\mathrm{+}}$ (A). Endogenous ${\mathrm{NH}}_{\mathrm{4}}^{\mathrm{+}}$ levels measured in the roots of 17-day-old plants (n > 10). Data represent means ± SE (n = 3) (B). qRT-PCR was performed to determine the expression levels of TaAMT genes in the roots. The level of TaAMT1;1 genes before ${\mathrm{NH}}_{\mathrm{4}}^{\mathrm{+}}$ treatment was defined as 1. The relative ratio shown is the ratio against 1 (C). Endogenous ${\mathrm{NH}}_{\mathrm{4}}^{\mathrm{+}}$ levels measured in the leaves of 17-day-old plants. Data represent means ± SE (n = 3) (D). qRT-PCR was performed to determine the expression levels of TaAMT genes in the leaves (E). The levels of TaAMT1;1 genes before ${\mathrm{NH}}_{\mathrm{4}}^{\mathrm{+}}$ treatment was defined as 1. The relative ratio shown is the ratio against 1. qRT-PCR was performed to determine the expression levels of TaAMT1 genes in the roots. The levels of TaAMT1;1 genes before N deprivation were defined as 1. The relative ratio shown is the ratio against 1. Significant differences at P < 0.05 level are indicated by different letters.