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. 2017 Sep 20;8:1614. doi: 10.3389/fpls.2017.01614

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Copyright © 2017 Daudu, Allion, Liesecke, Papon, Courdavault, Dugé de Bernonville, Mélin, Oudin, Clastre, Lanoue, Courtois, Pichon, Giron, Carpin, Giglioli-Guivarc’h, Crèche, Besseau and Glévarec.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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sln1Δ mutated yeast complementation assays with MdCHK. (A) Cytokinin-dependant growth phenotype of mutant yeast sln1Δ transformed with pYES2-MdCHK. Suspensions of transformed yeasts were spotted onto plates containing or not tZ (10 μ M). (B) Cytokinin binding specificity and sensitivity profiles of MdCHKs. MdCHK-complemented yeasts were grown in liquid culture medium containing different cytokinin types with different concentrations for 48 h. Minimum concentration inducing yeast growth is indicated for each cytokinin tested. iP, isopentenyladenine; tZ, trans-zeatin; cZ, cis-zeatin; DHZ, dihydrozeatin; iPA, isopentenyladenosine; tZR, trans-zeatin riboside; cZR, cis-zeatin riboside; DHZR, dihydrozeatin riboside; iPRMP, isopentenyladenosine 5′-monophosphate; tZRMP, trans-zeatin riboside 5′-monophosphate; cZRMP, cis-zeatin riboside 5′-monophosphate; DHZ, dihydrozeatin riboside 5′-monophosphate; tZOG, trans-zeatin O-glucoside; tZOGR; trans-zeatin O-glucoside riboside; tZ7G, trans-zeatin N7-glucoside; 2MeSiP, 2-methylthio-isopentyladenine; 2MeStZ, 2-methylthio-trans-zeatin; 2MeScZ, 2-methylthio-cis-zeatin; 2MeSiPA, 2-methylthio-isopentyladenosine; 2MeStZR, 2-methylthio-trans-zeatin riboside; 2MeScZR, 2-methylthio-cis-zeatin riboside. ^∗, no growth observed. Basal growth of MdCHK3a- and MdCHK3b-complemented yeast is also indicated (•).