FIGURE 4.

MdCHKs display various expression patterns in apple tree organs. Total transcript numbers of MdCHK2, MdCHK3a, MdCHK3b, MdCHK4a, and MdCHK4b were determined by real-time RT-PCR analyses performed on total RNA extracted from various M. domestica organs. R, roots; S, stems; L, leaves; FB, flower buds; F, flowers. Transcript abundance of each gene was normalized against EF1α reference gene. Data have been analyzed by ANOVA (p < 0.05), after running Shapiro–Wilk and Bartlett tests, followed by HSD Tukey test. Error bars were calculated from triplicates.