Fig.1.
Heterogeneity in Aβ1 - 42 binding and internalization. A) Only certain neurons in culture accumulate synthetic Aβ1 - 42 in a punctate pattern along the processes. Epifluorescent imaging of wt primary mouse neurons treated with 1 μM human Aβ1 - 42 for 24 h or 48 h. Labeling with a C-terminal specific Aβx - 42 antibody (left panels) showing both endogenous mouse Aβ42 and the added human synthetic Aβ1 - 42, displays two large neurons in each of these images. However, the exogenously added human Aβ1 - 42, recognized by antibody 6E10 (right panels), accumulates predominantly only in one of the two neurons, including their processes. Note the brighter labeling with the high affinity antibody 6E10 of only one of the two neurons in the right image panels. Scale bars 50 μm. B-C) Accumulation of exogenously added Aβ1 - 42 for 30 min is more pronounced in excitatory CamKII-positive compared to inhibitory GAD67-positive neurons. B) Aβ1 - 42 accumulation in some but not all, and not exclusively in, neurons labeled with CamKIIα. Higher magnification images (right) with Aβ1 - 42 in CamKIIα-positive synaptic terminals that appear more consistent with dendritic spines. C) Aβ1 - 42 was not seen accumulating in any GAD67-positive neurons.