Fig.3.
The pattern of Aβ accumulation in neurons is more consistent with dendritic labeling. A-D) Double-labeling with axonal tau-1 (A) and dendritic MAP2 (B) markers of primary neurons treated with 0.5 μM of fluorescently tagged Aβ1 - 42 (Aβ555) for 30 min. All images show the same field of view. Merged images of both tau-1, MAP2, Aβ1 - 42, and DAPI are shown in row C. The panel to the far right show higher magnification images of the middle panel. The pattern of Aβ labeling (shown separately in row D) is more consistent with that of dendritic MAP2 than tau-1 labeling. White arrows denote MAP2-positive dendrites accumulating Aβ555, which are not positive for tau-1. High magnification images (right) show tau-1-positive axons devoid of Aβ555. Scale bars 50 μm.