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. Author manuscript; available in PMC: 2017 Sep 25.
Published in final edited form as: Cell Rep. 2017 Aug 8;20(6):1295–1306. doi: 10.1016/j.celrep.2017.07.035

Figure 6. Constitutive Activation of the Sonic Hedgehog Pathways in Pdx1-Cre;αE-Catenin-KO Pancreas Prevents the Acquisition of an Endocrine Cell Phenotype.

Figure 6

The expression of genes positively regulating the activity of the SHH pathways is significantly upregulated in the pancreatic epithelium of Pdx1-Cre;αE-catenin-KO pancreas, both during development at E15.5 (A) and at birth day P0 (B).

(A and B) Data are shown as mean ± SEM from n = 4 independent experiments; *p < 0.05; **p < 0.001.

(C–M) Organ cultures of P0 pancreata from WT (C–E) and Pdx1-Cre;αE-catenin-KO mice (F–H) under control conditions. Addition of cyclopamine to organ cultures from Pdx1-Cre;αE-catenin-KO pancreata (I–K) rescued endocrine cell differentiation. Cyclopamine treatment normalizes the overall endocrine cell area (L) and reduces the frequency of proliferating Sox9+ cells to levels close to those measured in WT pancreata (M). qPCR data presented in (A) and (B) are from 4 independent experiments. Morphometric analysis on organ cultures (C–M) was performed on samples from n = 8 independent experiments. Reference bar, 50 µm.