Table 2.

Cytokine mRNA expression levels in whole brain homogenates.

Cyotkine	Day of life	CD		HFD
		Male	Female	Male	Female
IL-1β	0	1.01 ± 0.10	1.14 ± 0.27	1.77 ± 0.21	1.85 ± 0.15
	7	1.05 ± 0.13	1.02 ± 0.11	1.33 ± 0.21	0.73 ± 0.06#
	21	1.05 ± 0.21	1.02 ± 0.15	0.37 ± 0.11*	0.73 ± 0.02
TNFα	0	1.01 ± 0.09	1.13 ± 0.33	1.93 ± 0.33	2.39 ± 0.33*
	7	1.04 ± 0.16	1.05 ± 0.25	0.54 ± 0.12	0.58 ± 0.07
	21	1.02 ± 0.15	0.55 ± 0.15	0.89 ± 0.12	1.10 ± 0.03
TGFβ	0	1.07 ± 0.22	1.07 ± 0.20	0.91 ± 0.08	1.01 ± 0.08
	7	1.03 ± 0.11	1.02 ± 0.09	1.65 ± 0.30	1.77 ± 0.26
	21	1.05 ± 0.21	1.00 ± 0.06	1.46 ± 0.35	1.18 ± 0.05
Nurr77	0	1.18 ± 0.37	1.01 ± 0.11	1.44 ± 0.13	1.16 ± 0.17
	7	1.06 ± 0.15	1.07 ± 0.18	2.67 ± 0.45*#	1.02 ± 0.19
	21	1.02 ± 0.14	1.03 ± 0.16	3.91 ± 0.16*	2.70 ± 0.13*#
Nurr1	0	1.00 ± 0.04	1.08 ± 0.15	1.64 ± 0.20*	1.22 ± 0.16
	7	1.04 ± 0.12	1.03 ± 0.13	0.89 ± 0.11	0.91 ± 0.20
	21	1.01 ± 0.10	1.02 ± 0.13	0.95 ± 0.03	0.81 ± 0.05

Cytokines were measured in whole brain homogenates by RT-PCR. Data were analyzed by Multivariate regression and effects of diet (0.045) and time (0.000) were indicated. Between subject effects indicated an effect of sex (0.044) and time (0.000) for IL-1β; an effect of diet (0.017) and time (0.011) for Nurr77; and an effect of time for Nurr1 (0.022). Tukey’s post hoc analyses are indicated, p < 0.05, n = 3–4

^*denotes statistically different than CD same gender;

^#denotes statistically different than same diet different gender.