Table 3.

Iron metabolizing protein mRNA levels in whole brain homogenates.

	Day of life	CD		HFD		Two-Way ANOVA
		Male	Female	Male	Female
Hepcidin	0	1.02 ± 0.09	1.11 ± 0.21	1.56 ± 0.31	1.92 ± 0.43	Effect of diet
	7	1.03 ± 0.11	1.08 ± 0.19	1.46 ± 0.25	1.27 ± 0.21	Effect of diet
	21	1.04 ± 0.20	1.02 ± 0.16	1.17 ± 0.07	0.93 ± 0.09
Ferroportin	0	1.01 ± 0.06	1.07 ± 0.15	1.30 ± 0.13	1.43 ± 0.16	Effect of diet
	7	1.01 ± 0.05	1.01 ± 0.05	0.53 ± 0.04*	0.75 ± 0.12	Effect of diet
	21	1.02 ± 0.15	1.05 ± 0.22	1.08 ± 0.09	1.16 ± 0.14
l-ferritin	0	1.02 ± 0.08	1.00 ± 0.04	1.49 ± 1.23	1.23 ± 0.11	Effect of diet
	7	1.00 ± 0.04	1.02 ± 0.08	0.54 ± 0.06*	0.68 ± 0.14	Effect of diet
	21	1.02 ± 0.15	1.00 ± 0.04	0.96 ± 0.06	1.11 ± 0.03

Iron regulatory proteins were measured in whole brain homogenates by RT-PCR. Data were analyzed by Multivariate regression using diet, gender, and time as co-variates and no overall effects were indicated. Data within day of life was analyzed by two-way ANOVA and Tukey’s post hoc, p < 0.05, n = 3–4. Multivariate analysis indicated significant differences in hepcidin. Effects across sex and diets within a time are noted on the table.

^*denotes statistically different than CD same gender;

^#denotes statistically different than same diet different gender.