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. 2016 Jan 26;3(1):6. doi: 10.3390/jcdd3010006

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© 2016 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Subcellular localization of the Gatad1 protein in the embryonic zebrafish heart. After the myl7:gatad1-GFP construct was injected into 1-cell staged embryos, hearts from 2 dpf embryos were dissected for immunostaining and imaging. (A) Gatad1-GFP shows strong expression in nuclei and relatively weak expression in myofibrils, overlapping with Actin as revealed by phalloidin staining; (B) Gatad1-GFP partially overlaps with Z-discs marked by Actinin (arrows); (C) Gatad1-GFP forms alternatively striated patterns with M-line marked by Myomesin (arrowheads). * Nucleus; Scale bar 5 μm.