(A) Calibration result of leave-one-out cross-validation for the calibration set (n = 150, including 50 HL60 cells, 50 K562 cells and 50 BMC). The RMSE value is 0.130, and the correlation coefficients square value is 0.974. (B) Prediction results of partial least squares algorithm for the test set (n = 30, including 10 HL60 cells, 10 K562 cells and 10 BMC). The red arrow indicates the misjudged sample (sample 6).