Fig. 2.

A three amino acid mutation in the SRM of Sall1 causes renal hypoplasia. (A) Schematic of the Sall1 locus, the targeting vector and the ΔSRM mutant allele. The targeting vector introduced a three-amino acid mutation into exon I of Sall1, a LoxP site (black arrowhead) and a neomycin cassette (Neo) flanked by Frt sites (white arrowheads) after this region. Deletion of the neomycin cassette by Flp recombinase yields the targeted ΔSRM locus. (B) E11.5 wild-type and homozygous ΔSRM mutant embryos. (C) Weanling (21 day old) wild-type and homozygous ΔSRM mutant mice and their kidneys. The mutant kidney is hypoplastic with cysts visible.