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. 2017 Aug 2;9(8):1885–1897. doi: 10.18632/aging.101271

Figure 4. MiR‐338 reduced the binding between PKM2 and β‐catenin to repress β‐catenin transcriptional activity.

Figure 4

(A) Co‐IP assay to investigate the effect of miR‐338 on the binding between PKM2 and β‐catenin. (B) U87 and SNB19 cells were transiently transfected (24 h) with the pGL4.74 plasmid and co‐transfected with pGL4.75. Cells were then treated as indicated for 48 h. Both firefly and Renilla luciferase activities were calculated and recorded as fold‐induction (P<0.05, P<0.05). (C) Cell cycle analysis of miR‐Scr/miR‐338‐transfected cells treated with EGF, and overview of the cell cycle. (D) Immunostaining of β‐catenin location after miR‐Scr/miR‐338‐ transfected cells were treated with EGF.