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. 2017 Sep 15;220(18):3238–3247. doi: 10.1242/jeb.159665

Fig. 3.

Fig. 3.

Natural variation in P. pacificus responses to the embryocide ZTDO. Early-stage embryos were exposed to 10 µl of 0.5% ZTDO or 100% ethanol suspended from plate lids for 4 h. Strains from the Pacific Rim – PS1843 (Washington), RLH163 (California), RS5194 (Japan), RS5186 (Japan) – resemble the reference strain PS312 (California). Strains from La Reunion (RS5419, RS5413, RSB001) and Bolivia (RS5278) are significantly less sensitive to ZTDO. Caenorhabditis elegans N2 do not respond to 0.5% ZTDO. The difference between the ethanol control and ZTDO was significant (two-tailed unpaired t-test, P<0.05), except for RSB001 and N2. Significant difference from PS312 was determined by one-way ANOVA followed by Dunnett's post hoc test (***P<0.0001). N (n): 10 (926), 10 (948), 15 (2894), 15 (3771), 15 (1899), 15 (2246), 15 (1678), 15 (1514), 15 (1482), 15 (2194), 15 (1965), 15 (2640), 15 (2735), 15 (2844), 15 (2077), 15 (2011), 15 (3497), 15 (3275), 15 (4335), 10 (3098). Error bars denote ±s.e.m.