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. 2017 Sep 15;220(18):3238–3247. doi: 10.1242/jeb.159665

Fig. 5.

Fig. 5.

Aberrant development in ZTDO-exposed P. pacificus embryos. Representative images of early-stage embryos exposed to 10 µl of 0.5% ZTDO suspended from plate lids for 4 h immediately following egg synchronization. The formation of a cavity-like structure for all embryonic stages increased with incubation time beyond the normal 26 h. (A) The ‘bean’ stage is the least developed of the zombryo stages, representing 26 and 32% of the total viewed at 26 and 48 h ex utero. (B) A ‘peanut’ stage embryo with an anterior cavity viewed at 48 h ex utero. (C) A ‘peanut’ stage embryo with a mid-body cavity viewed at 48 h ex utero. (D) A 2-fold stage embryo with an open anterior cavity viewed at 26 h ex utero. (E) A 3-fold stage embryo with a mid-body cavity viewed at 26 h ex utero. The asterisk denotes the cavity in each panel. Scale bar of 25 µm in B represents the same magnification for all images. N (n): 3 (115) for 26 h, 3 (93) for 48 h.