Figure 3.

Purification and characterization of recombinant R. sanguineus evasin-4. A, size exclusion chromatogram for purification of evasin-4 (after His₆ tag removal) with non-reducing SDS-PAGE of fractions (Fr.) spanning the main peak (boxed on the chromatogram); the molecular weight marker used was Bio-Rad Precision Plus Protein^TM unstained standards. B, fluorescence anisotropy-binding curves showing displacement of a fluorescent CCR2-derived sulfopeptide from each of six chemokines using purified recombinant evasin-4. Data plotted are the averages of three independent experiments, each recorded in duplicate, and error bars represent the S.E. Solid lines are fitted binding displacement curves.