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. 2017 Aug 4;292(38):15670–15680. doi: 10.1074/jbc.M117.807255

Figure 5.

Figure 5.

Inhibition of chemokine activity by purified evasins. Shown are concentration-response curves for inhibition of the chemokines MCP-1 (10 nm) (A) and MCP-2 (100 nm) (B) acting at the receptor CCR2 and for inhibition of the chemokines eotaxin-1 (100 nm) (C) and eotaxin-2 (100 nm) (D) acting at the receptor CCR3. Chemokine activity was detected as the ability of the chemokine to inhibit forskolin (FSK)-induced production of cAMP as detected via a BRET sensor (see “Experimental procedures” for details); thus, evasins inhibit the cAMP-inhibitory activity of the chemokines. Data represent the average ± S.E. (error bars) of values from three independent experiments, each recorded in duplicate.