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. 2017 Aug 9;292(38):15814–15825. doi: 10.1074/jbc.M117.777532

Figure 2.

Figure 2.

Removal of Sufu in CNC does not affect migration, but does affect condensation and ossification of CNC-derived mesenchymal cells. A–H, X-gal staining showing the migration of CNC-derived mesenchymal cells to the presumptive frontal primordium in R26R;Sufufx/fx;Wnt1-Cre (R26R;SufuWnt1Cre) embryos and littermate control mice (R26R;Sufufx/+;Wnt1-Cre, n = 3). I–N, H&E staining showing the condensation of mesenchymal cells in the frontal primordium both in littermate wild type and Sufufx/fx;Wnt1-Cre (SufuWnt1Cre) mutant (n = 3). O–R, von Kossa staining showing the mineralization of frontal bones in E14.5 in littermate wild type versus Sufufx/fx;Wnt1-Cre (SufuWnt1Cre) samples (n = 3). S and T, In situ hybridization showing the expression of mineralization marker OPN in the wild-type frontal bone but not in the littermate Sufu mutant (n = 2). Arrowhead, the presumptive frontal primordium. Scale bars, 1 mm (A and B) and 200 μm (C–T).