Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 9;292(38):15916–15926. doi: 10.1074/jbc.M117.796771

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

PMC Copyright notice

Figure 4. — Effect of the miR-125b/SUV39H1 axis on H3K9me3 levels and heterochromatin deposition. A and B, immunoblotting analysis of H3K9me3 levels after knockdown of both miR-125b and SUV39H1 in BFFs (A) or SCNT embryos (B). Protein expression levels were normalized to histone H3 levels. C, qPCR analysis of satellite expression in SCNT embryos after knockdown of both miR-125b and SUV39H1 (*, p < 0.05). The GAPDH level was set to 1.0. Bars indicate the S.E. D and E, immunoblotting analysis of H3K9me3 levels after overexpressing both miR-125b and SUV39H1 in BFFs (D) and SCNT embryos (E). Protein expression levels were normalized to the histone H3 levels. F, qPCR analysis of satellite expression in SCNT embryos after overexpressing both miR-125b and SUV39H1 (*, p < 0.05). The GAPDH level was set to 1.0. Bars indicate the S.E.