Figure 6.

Ror1 is required for TNF-α-dependent proliferation of C2C12 cells. A, expression levels of Ror1 mRNA in C2C12 cells, transiently transfected with either Ror1 siRNAs (Ror1#1 and Ror1#2) or control siRNA (Ctrl), followed by stimulation with TNF-α (10 ng/ml) for 24 h, were measured by qRT-PCR analysis. Relative expression values were determined by defining expression level of Ror1 in cells transfected with control siRNA, followed by treatment with vehicle alone as 1. Data are expressed as mean ± S.D. (n = 3). (*, p < 0.05; **, p < 0.01, n.s. (not significant), Bonferroni's post hoc test.) B, C2C12 cells, transiently transfected with either Ror1 siRNAs (Ror1#1 and Ror1#2) or control siRNA (Ctrl), followed by stimulation with TNF-α (10 ng/ml) for 24 h, were visualized by staining with anti-Ki67 antibody (green) and DAPI (blue). Scale bar, 100 μm. C, proportions of Ki67-positive C2C12 cells were quantified. Data are expressed as mean ± S.D. (n = 3). (**, p < 0.01, n.s. (not significant), Bonferroni's post hoc test.)