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. 2017 Aug 9;292(38):15952–15963. doi: 10.1074/jbc.M117.792010

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Figure 5. — Wave3 was an Hsp70 downstream mediator responsible for p63α promotion of BC invasion. A, T24T(Vector), T24T(p63α/Nonsense), T24T(p63α/shHsp70-1), and T24T(p63α/shHsp70–2) cells were extracted upon the density reaching 80–90%, and the cell extracts were subjected to Western blotting with the specific antibodies as indicated. GAPDH was used as a protein-loading control. B and C, the invasion abilities of T24T(p63α/Nonsense), T24T(p63α/shHsp70-1), and T24T(p63α/shHsp70–2) cells were subjected to a transwell invasion assay. B, the invasion rate was normalized with the insert control according to the manufacturer's instruction, and the results are presented as relative invasion cells. C, the asterisk (*) indicates a significant difference of invasion abilities between T24T(p63α/Nonsense) and T24T(p63α/shHsp70) cells (p < 0.05). D, cell proliferation was evaluated by ATG assay. E, T24T(p63α/Nonsense) and T24T(p63α/shHsp70-1) cells were treated with 50 μg/ml cycloheximide (CHX) for the indicated time periods, and the cell extracts were subjected to Western blotting to analyze Wasf3 and Hsp70 protein degradation rates. β-Actin was used as a protein loading control. F, Wasf3 protein degradations from three independent experiments were analyzed and presented. The asterisk (*) indicates a significant difference between the indicated two transfectants.