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. 2017 Sep 15;130(18):3173–3185. doi: 10.1242/jcs.194571

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 8. — The purified novel compound derived from IS1 causes a rounded cell phenotype. (A) UV and visible absorbance spectrum of the IS1 compound. (B) Mass spectrometry data for compound purified from IS1 [M+H]⁺. (C) SAK950 control (left panel), SAK950 cells from the plug assay with IS1 (middle panel), and SAK950 cells from filter disc assays with ‘peak-picked’ purified IS1 compound (right panel). (D) Representative cells of SAK950 control (left panels) and SAK950+peak picked IS1 from B shown at higher magnification (right panels). Scale bars: 10 µm.