Fig. 2.
Cdc42-null fibroblasts fail to protrude in response to optogenetic ITSN1 localization. (A) Representative western blot analysis of Cdc42 protein levels in MEFs containing a single floxed Cdc42 allele (Cdc42f/−) and either untreated or transduced with Adeno-Cre virus (to give Cdc42−/− cells). (B) Representative fluorescence micrographs of live fibroblasts from A being optogenetically activated to recruit ITSN1-micro to a ROI. Right panels are kymographs along the yellow line. Blue arrows denote activated areas. Scale bars: 50 µm (fluorescence images); 5 µm and 250 s (kymographs). (C) Mean distance for the ITSN1 DH/PH-induced protrusions for the denoted genotype as determined from the kymographs. *P<0.05. Refer to Table S2 for experimental details.