Figure 2.

Changes in the gut microbiota of pet store mice and their offspring associated with quarantine and antibiotic treatment. Stacked bar charts showing relative abundance of operational taxonomic units (OTUs) detected in the feces of a second cohort of adult mice purchased from a pet store in Columbia, MO and two litters born to those mice following 10 weeks in quarantine and treatment with azithromycin (a); Principal component analysis plot showing differences in β-diversity of fecal communities in a second cohort of adult pet store mice immediately upon arrival (n = 19), following 6 weeks of quarantine (post-Q, n = 20), adult F1 mice from two litters born to the pet store mice treated with azithromycin; and adult BALB/c mice purchased from Jackson (n = 10) or Harlan (n = 10) (b); bar chart showing the mean (± standard deviation) number of OTUs detected in feces from each group. Like letters indicate significant (p < 0.05) differences as determined via Kruskal-Wallis one-way ANOVA on ranks (c).