Table 1.

Candidate Dcp-1 interactors and substrates identified by mass spectrometry. Symbols, CG numbers and molecular functions are from FlyBase.

FlybaseSymbol	CG Number	Gene Ontology molecular function	Mean log(e)	Mean unique peps	UniProt human gene name	# of Expts observed
14–3–3ζ	CG17870	Protein binding, protein heterodimerization activity, protein homodimerization activity	−9.6	1.75	YWHAZ	4
eEF1α1	CG8280	Translation elongation factor activity	−27	3.75	EEF1A1	4
Hsc70–4	CG4264	Chaperone binding	−70	8.50	HSPA8	4
Hsp83	CG1242	ATPase activity, coupled	−32	4.75	HSP90AA1	4
Jafrac1	CG1633	Thioredoxin peroxidase activity	−30	4.00	PRDX1	4
14–3–3ε	CG31196	Protein binding; protein heterodimerization activity	−23	3.33	YWHAE	3
blw	CG3612	Hydrogen exporting ATPase activity; phosphorylative mechanism	−16	2.67	ATP5A1	3
CCT2	CG7033	Unfolded protein binding; ATP binding	−6.5	1.67	CCT2	3
Clic	CG10997	Calcium ion binding; chloride channel activity; lipid binding	−7.2	1.67	CLIC2	3
eEF1β	CG6341	Translation elongation factor activity	−10	2.00	EEF1B2	3
Rack1	CG7111	Protein kinase c binding	−3.8	1.33	RACK1	3
sesBa	CG16944	ATP:ADP antiporter activity	−27	4.00	ANT2	3
sgl	CG10072	UDP-glucose 6-dehydrogenase activity	−8.1	1.67	UGDH	3
TER94	CG2331	ATPase activity; golgi & ER organization	−16	2.67	VCP	3
Uba1	CG1782	Ubiquitin activating enzyme activity	−43	5.67	UBA1	3
ATPsynβ	CG11154	Hydrogen exporting ATPase activity; phosphorylative mechanism	−3.1	1.00	ATP5B	2
eEF1γ	CG11901	Translation elongation factor activity	−5.6	1.50	EEF1G	2
eIF4a	CG9075	Translation initiation factor activity; RNA helicase activity	−17	3.00	EIF4A1	2
Hsc70Cb	CG6603	Chaperone binding	−19	3.00	HSPA4	2
Hsp60A	CG12101	Unfolded protein binding	−4.7	1.50	HSPD1	2
Hsp70Aa	CG31366	ATP binding, response to hypoxia	−19	1.00	HSPA1A/1B/1L	2
Mi-2	CG8103	Protein binding; nucleosome-dependent ATPase activity; chromatin binding	−6.1	1.50	CHD3	2
REG	CG1591	Endopeptidase inhibitor activity; endopeptidase activator activity	−3.4	1.00	PSME3	2
sta	CG14792	Structural constituent of ribosome	−5.8	1.50	RPSA	2

Notes. The mean number of unique peptides that corresponded to each gene and the mean X!Tandem log (e) score for the peptides identified are listed. The human gene names were determined from a BLAST analysis of the Drosophila genes against the human UniProt database. The list is ordered by number of observations made from 4 independent immuno-affinity purifications of the Dcp-1 protein (Expts) with the most being 4 out of 4 experiments and the least being 2. See Table S1 for all raw values.

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