Figure 5.

Endosomes are involved in autophagosome formation in SKP/SKO-induced reprogramming. (A) Chimeric markers targeted to different organelles were expressed during SKP/SKO reprogramming at d 5 (top to bottom: early endosomes (EE, RAB5A), late endosomes (LE, RAB7), recycling endosomes (RE, RAB11A), Golgi (B4GALT), and endoplasmic reticulum (ER); scale bar: 10 µm (B) Quantification of GFP-LC3B overlap with different organelle membrane markers. (C) Persistent signal of early endosome membrane on an autophagosome by a photobleaching outside the ROI assay during SKP/SKO reprogramming at d 5. All fluorescence outside the ROI (marked by square) was photobleached. The fluorescence within ROI was monitored after photobleaching. Scale bars: 5 µm, upper panel; 1 µm, lower panel.