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Decreased levels of PARP-1 activity do not affect H3S10 phosphorylation by JIL-1. (A) Polytene squash preparations from wild-type and hypomorphic homozygous ParpC03256 mutant larvae labeled with antibody to H3S10ph (red), with DNA (Hoechst) in blue or gray. (B) Immunoblots of protein extracts from salivary glands of wild-type and hypomorphic homozygous ParpC03256 mutant larvae labeled with antibody to H3S10ph. Labeling with histone H3 antibody provided a loading control.
