Fig. 6.

RP epithelium fails to activate LHX3 and LHX4 expression in Hesx1^Cre/+;Shh^fl/− mutants. Immunofluorescence and in situ hybridisation on mid-sagittal sections on Hesx1^Cre/+;Shh^fl/− and control embryos against RP progenitor markers. Markers and stages are indicated. (A-L) Single immunofluorescent staining showing that while PITX1 expression is maintained, LHX3 is undetectable in the RP of the mutants compared with control embryos from 9.5 to 11.5 dpc (arrowheads). (M-P) In situ hybridisation confirms the lack of Lhx3 and Lhx4 expression in the mutant RP at 10.5 dpc (arrowheads). (Q-X) Transcripts for other RP markers such as Pitx2, Six3, Pax6 and Wnt4 are detected in the developing mutant and control RP (arrowheads). RP, Rathke's pouch; PH, posterior hypothalamus; AH, anterior hypothalamus. Scale bar: 100 μm.