Fig. 4.

Microtubule patterns and FB partitioning in R. sp. SB347 near relatives. (A) Molecular phylogeny of near relatives (Kanzaki et al., 2017). (B-F) Fixed spermatocytes and sperm from R. sp. SB372 (B,C), R. sp. JU1783 (D,E), and R. axei (F) labeled with DAPI (blue) and antibodies against either α-tubulin (T) or MSP (FBs) in same cell (B,C) or same-stage (F) DIC images in the left column. Cell stages as in Fig. 2. R. axei sperm that have (S*) or have not (S**) separated from each other before secondarily separating from residual body components are also shown. Arrows label lagging X chromatid (yellow), X-bearing sperm (white), RBs with or without a chromatin mass (green), FB clearing (purple), and chromosomes compacting into a single mass (orange). Scale bars: 5 µm. Sperm size measurements calculated from >5 gonads, 20-30 sperm/gonad.