% cells in DNA content gate | Purity of sorted germ cell populations (%) | |||||||||||
Species | % Singlets | % Live cells | 1C | 2C | 4C | Spg | Spc I | Spc II | Spd | rSpd | eSpd | |
Mouse | 98.4 | 92.5 | 34.7 | 3.9 | 3.72 | 74 | 82 | 87.5 | 95.2 | 95* | 92* | |
Rat | 95.7 | 93.8 | 37.7 | 5.3 | 5.4 | 83 | 81 | 82 | 87 | . | . | |
Guinea Pig | 96.2 | 92.1 | 39.3 | 7.6 | 5.8 | 48 | 68.7 | 85 | 87 | . | . | |
Dog | 97.9 | 86.5 | 16.4 | 3 | 0.5 | 78 | . | 87 | . | 91 | 81 | |
Mini Pig | 95.9 | 93.2 | 26.9 | 6.4 | 3.5 | 49 | 52 | 82 | 92 | . | . | |
* Obtained by enzymatic dissociation and gated based on FSC&SSC parameters (from Lima et al. 2016, with permission) |