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. 2017 Jul 12;(125):55913. doi: 10.3791/55913
% cells in DNA content gate Purity of sorted germ cell populations (%)
Species % Singlets % Live cells 1C 2C 4C Spg Spc I Spc II Spd rSpd eSpd
Mouse 98.4 92.5 34.7 3.9 3.72 74 82 87.5 95.2 95* 92*
Rat 95.7 93.8 37.7 5.3 5.4 83 81 82 87 . .
Guinea Pig 96.2 92.1 39.3 7.6 5.8 48 68.7 85 87 . .
Dog 97.9 86.5 16.4 3 0.5 78 . 87 . 91 81
Mini Pig 95.9 93.2 26.9 6.4 3.5 49 52 82 92 . .
* Obtained by enzymatic dissociation and gated based on FSC&SSC parameters (from Lima et al. 2016, with permission)