Table 1.
Integrins Identified in Trabecular Meshwork and Optic Nerve Head in vitro and in situ (Zhou et al., 1995; Tervo et al., 1995; Diskin et al., 2009). The identity of these integrins is based onknown pairings between subunits (Humphries et al., 2006).
| Integrin | Location found |
Ligands | Consensus Motif |
Detection method(s) |
References |
|---|---|---|---|---|---|
| α1β1 | TM | Collagens (I, IV, IX), Laminin,Galectin-8 | GFOGER | IHC in situ and IP | (Zhou et al., 1999a) |
| α2β1 | TM and ONH | Collagens (I, III, IV, IX), Laminin, E-cadherin, TSP-1, Tenascin-C | GFOGER | IP and RT-PCR | (Zhou et al., 1999a; Morrison, 2006) |
| α3β1 | TM and ONH | Laminin, TSP-1, Galectin-8 | Not Identified | IHC/IF in situ and IP | (Tervo et al., 1995; Zhou et al., 1999a; Morrison, 2006) |
| α4β1 | TM and ONH | Fibronectin, VCAM-1, ICAM-4, TSP-1, Osteopontin, ADAM2 | LI/-D/E-V/S/T-P/S (LDV) | IHC in situ, IF in tissue culture, Flow, and RT-PCR | (Zhou et al., 1999a; Morrison, 2006; Schwinn et al., 2010; Faralli et al., 2011; Neumann et al., 2014) |
| α5β1 | TM and ONH | Fibronectin, Fibrillin, Osteopontin, TSP-1, Galectin-8, ADAM9,12,15,23 | RGD | IF in tissue culture, IP and RT-PCR | (Zhou et al., 1999a; Morrison, 2006; Junglass et al., 2009; Faralli et al., 2011; Neumann et al., 2014) |
| α6β1 | TM and ONH | Laminin, TSP-1, ADAM9,12,15,23 | Not Identified | IHC/IF in situ and IP | (Tervo et al., 1995; Zhou et al., 1999a; Morrison, 2006) |
| α6β4* | TM and ONH | Laminin | Not Identified | IHC/IF in situ, IP | (Tervo et al., 1995; Zhou et al., 1999a; Morrison, 2006; Perkumas and Stamer, 2012) |
| α9β1 | TM and ONH | Fibronectin | EDGIHEL | RT-PCR | (Neumann et al., 2014; Park et al., 2014) |
| αvβ1 | TM and ONH | Fibronectin, Vitronectin, Osteopontin, ADAM9,12,15,23, Galectin-8, LAP-TGF-β | RGD | IHC/IF in situ, RT-PCR, IP | (Zhou et al., 1999a; Morrison, 2006; Junglass et al., 2009) |
| αvβ3** | TM and ONH | Fibronectin, Vitronectin, TSP-1,2, tenascin, and Collagen IV, CTGF, MMP-2, PECAM-1, Fibrillin, LAP-TGF-β, Osteopontin | RGD | IHC/IF in situ, IF in tissue culture, Flow IP and PCR | (Tervo et al., 1995; Zhou et al., 1999a; Morrison, 2006; Filla et al., 2006; Filla et al., 2009; Filla et al., 2011; Gagen et al., 2013; Faralli et al., 2013; Pattabiraman and Rao, 2015) |
| αvβ5 | TM and ONH | Fibronectin, Vitronectin, LAP-TGF-β, Osteopontin, ADAM9,12,15,23 | RGD | IF in tissue culture and PCR | (Tervo et al., 1995; Zhou et al., 1999a; Morrison, 2006; Gagen et al., 2013; Neumann et al., 2014) |
*
β4 subunit has not been detectedinTM cell cultures.
**
The splice variants for β3A and β3B were detected in TM cell cultures by PCR (unpublished data). Splice variants for β1, β5, α3 and α6 integrin subunits have not been detected in TM or ONH.