Table 2.
Specific multilocus sequence typing (MLST) for Kingella kingae performed by Sanger sequencing method on DNA directly extracted from 11 oropharyngeal specimens (=11 children) with no prior bacterial isolation allowed to detect K. kingae clones belonging to ST-6 in 6 children, ST-25 in 2, and possible new STs in 3, namely ST-67, ST-68, and ST-69
| No. | Age (mo) | Year | Syndrome | Country/region | abcZ | adk | aroE | cpn60 | gdh/zwf | recA | ST | STc |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1572468 | 17 | 2016 | OAI | France | 5 | 2 | 4 | 5 | 5 | 1 | 6 | 6 |
| 1980738 | 16 | 2016 | OAI | France | 5 | 2 | 4 | 5* | 5 | 1 | 6 | 6 |
| 1956884 | 18 | 2016 | OAI | France | 5 | 2 | 4 | 5 | 5 | 1 | 6 | 6 |
| 1882247 | 16 | 2016 | OAI | France | 5 | 2 | 4 | 5 | 5 | 1 | 6 | 6 |
| 1815589 | 12 | 2016 | OAI | France | 5* | 2 | 4* | 5 | 5* | 1* | 6 | 6 |
| 6847254 | 8 | 2016 | OAI | France | 5 | 2 | 4* | 5* | 5 | 1* | 6 | 6 |
| 1541670 | 11 | 2016 | OAI | France | 7 | 2 | 6 | 2 | 2 | 2 | 25 | 25 |
| 0990626 | 28 | 2015 | OAI | France | 7* | 2 | 6 | 2 | 2 | 2 | 25 | 25 |
| 1822057 | 7 | 2016 | OAI | France | 1 | 2 | 6 | 2* | 1* | 14* | 69 | … |
| 1730798 | 33 | 2013 | AC | French Guiana | 5 | 2 | 3* | 2 | 2 | 2 | 68 | … |
| 1746575 | 8 | 2013 | AC | French Guiana | 5* | 2 | 6* | 11* | 9* | 1* | 67 | … |
*: detection of SNPs OAI osteoarticular infections, AC asymptomatical carriage, mo: months …: not defined
Data referring to the second epidemic case of the Châteauneuf-Grasse K. kingae outbreak 2016 are indicated in bold