Figure 2. Characterization of TEMPO.

Magnetic nanoparticle labeled cultured tumor cells positive for YFP were separated from mouse leukocytes using TEMPO, and quantified by flow cytometry a. before and b. after filtration. c. Very high depletion of leukocytes was achieved ζ >10⁴ at flow rates Φ> 10 mL h⁻¹ using N = 6 filters in series. Inset: depletion ζ depended on flow rate Φ as a power law. d. Depletion at 10 mL/hr could be improved exponentially in N by placing N filters in series. Thus, flow rate can be further increased beyond 10 mL/hr and depletion conserved, by continuing to add filters in series.