TABLE 1.

Timeline of procedures used in the present study.

Day(s)	Period	Procedures
Days 1–5	Social Bonding	Initial pairing of male and female prairie voles in clean, neutral cages
Days 6–10	Continued Pairing or Social Isolation	Paired voles remain in paired cages; isolated voles are separated into individual cages (cage changes matched)
Days 11–20	Chronic Mild Stress	Paired and isolated voles are exposed to 10 days of chronic mild stressors (details in Figure 1; cage changes matched)
Day 21	Tail Suspension Test	Paired and isolated voles are exposed to a 5-minute tail suspension test, followed by replacement in the home (paired or isolated) cage
Day 22	Forced Swim Test	Paired and isolated voles are exposed to a 5-minute forced swim test, followed by replacement in the home (paired or isolated) cage and heat lamp for 15 minutes
Day 25	Sample Collection	Blood collection from all paired and isolated voles