Growth medium (GM):	Concentration		Differentiation medium (DM):	Concentration
Ham's F10			DMEM
FCS	15%		bovine serum albumin	0.5 mg/ml
bovine serum albumin	0.5 mg/ml		epidermal growth factor	10 ng/ml
fetuin	0.5 mg/ml		insulin	0.01 mg/ml
epidermal growth factor	10 ng/ml		creatine	1 mM
dexamethasone	0.39 µg/ml		pyruvate	100 µg/ml
insulin	0.04 mg/ml		uridine	50 µg/ml
creatine	1 mM		gentamycin	10 µg/ml
pyruvate	100 µg/ml
uridine	50 µg/ml
gentamycin	5 µg/ml