Extended Data Figure 7. NAA responses.

a, Systemic autophagy grading upon tumour intrinsic inhibition of JNK signalling in the EAD. Values depict mean ± s.e.m. of three independent pooled experiments. n = 7 (Ras^V12scrib^−/−) or 11 (>bsk^DNRas^V12scrib^−/−) animals. b–k, Representative confocal images, quantified in Extended Data Fig. 8k–m. NAA is downstream of Fos and Ets21c transcription factor activity (b). Expression of bsk^DN suppresses NAA (c). Clonal overexpression of yki is sufficient to trigger autophagy in neighbouring cells (d). Knockdown of yki suppresses NAA (e). Reduction of tumour growth by disrupted PI3K-I signalling does not affect NAA induction (f). ImpL2 is neither sufficient nor necessary for NAA (g, h). Upd3 cooperates with Ras^V12 for NAA induction (i).. Removal of JAK/STAT signalling in neighbouring tissue does not suppress NAA (j). Quantification of relative 3 × ChAtg8a-intensities of indicated genotypes (k). Data are mean ± s.e.m. from three independent pooled experiments. n = 38 (Ras^V12scrib^−/−), n = 24 (Ras^V12egr^−/−scrib^−/−), 18 (Ras^V12scrib^−/−, >bsk^DN), n = 17 (Ras^V12scrib^−/−kay^−/−), 29 (Ras^V12ets21c^RNAiscrib^−/−fos^RNAi),n = 34 (Ras^V12dome^DNscrib^−/−),n = 5 (Ras^V12upd⁺ctrl) or n = 9 (yki^actctrl) discs. l, Relative 3× ChAt8ag intensities of indicated genotypes. Data are mean ± s.e.m. from three independent pooled experiments, n = 15 (Ras^V12dlg^RNAi),n = 11 (Ras^V12 dlg^RNAisd^RNAi),n = 2 (Ras^V12dlg^RNAibsk^DN), n = 10 (Ras^V12dlg^RNAiyki^RNAi), n = 7 (Ras^V12dlg^RNAi ImpL2^RNAi) or n = 5 (Ras^V12 dlg^RNAi dp^110DN) discs. m, Quantification of relative ChAtg8a intensities of indicated genotypes. Data are mean ± s.e.m. of three independent pooled experiments. n = 24 (Ras^V12ctrl),n = 20 (Ras^V12scrib^−/−), n = 9 (ImpL2⁺Ras^V12ctrl), n = 12 (upd3Ras^V12ctrl) or 15 (Ras^V12scrib^−/−//stat) discs. Scale bars, 50 μm (b–j). *P = 0.0127; ns, not significant by unpaired two-tailed t-test (a). *P ≤ 0.05, **P ≤ 0.01, ***P < 0.0001 by one-way ANOVA with Dunnett’s correction (k–m).